Sanger Sequencing Services

The High-Throughput Sequencing team provides a vast array of services for high-throughput sequencing projects, and customers are allowed to submit samples starting at any stage in the Sanger sequencing process. The unit can prepare both custom library construction for EST (primary and normalized) and BAC shotgun library sequencing. Please see our Library Services page for more information. For the Sanger dideoxy-terminator sequencing, we can take a project from the beginning stages of plating bacteria, colony picking the library, then inoculating and culturing the bacteria using the QpixII. Duplicate glycerol stocks are provided for customers. The plasmid preps used for sequencing are purified using either the Qiagen 9600 BioRobot or the cost-effective Heat Lysis method. Conditions for each project are optimized to produce the best sequencing data. Primers for sequencing are designed and made in house or can be supplied by the customer, and the Robbins Hydra is utilized for precise reaction set-ups. Cycling conditions are optimized on the BioRad/MJ Research DNA tetrads. Big Dye terminator chemistry is used for Sanger sequencing reactions and all sequencing is performed on the ABI 3730XL capillary systems. All 384- and 96-well format plates are labeled with a barcode, and a high throughput laboratory information management system (HTLIMS) is used to track sample flow from plate submission through sequencing.

Sanger Sequencing Pricing

Please contact Alvaro Hernandez, Director of DNA Services ( aghernan@illinois.edu ) at 217-244-3480 or Chris Wright, Assistant Director of DNA Services ( clwright@illinois.edu) at 217-333-4372 to discuss ways the staff can be of assistance in achieving your project goals and/or to provide a quote for your project.

High-Throughput Sequencing and Genotyping Unit
Director: Alvaro Hernandez, Ph.D.
340 Edward R. Madigan Laboratory, 1201 W. Gregory Drive, Urbana, IL 61801
Phone: (217) 244-3480      FAX: (217) 265-5066      Email: aghernan@illinois.edu