Roche 454 GS-FLX Titanium System:Ultra-high-throughput sequencing for genomic DNA, cDNA, environmental samples (metagenomes), PCR amplicons, amplified tag fragments (microRNA, ChiP-derived, methlyation enriched, captured sequences, SAGE, etc.), BAC clones or pools, and more. For descriptions and guidelines for Roche/454 sample submission, please visit our Roche/454 Sequencing Page.

454 Library Prep Services:

• RNA extraction per tissue: $200
• cDNA synthesis and normalization: $3,000
• Amplicon (PCR products) quantity and quality measurement(for up to twelve samples): $200

• 454 Titanium library preparation (nebulization and adaptor ligation): $600 for the first library, $400 for any additional if prepared at the same time. MIDs (tags) can be included at no charge if requested.

• 454 Titanium Paired End (3kb, 8kb, or 20kb) library preparation: $1000 for the first library, $500 for any additional library prepared from the same sample. $800 for any additional library prepared from different samples if prepared at the same time. MIDs (tags) can be included at no charge if requested.

454 Titanium Sequencing Services:

• Titration (Required for projects sequencing ½ bulk PTP or more): $2,200

454 FLX Sequencing Services (Amplicons only):

Illumina Genome Analyzer IIx system : ultra high-throughput sequencing for mRNA, digital gene expression (NlaIII or DpnII kits), genomic DNA, Chip DNA, small RNAs.

• gDNA, mRNA, small RNA: $700 for first sample, $400 for each additional up to 7 samples done concurrently

• Digital Gene Expression (NlaIII and DpnII): $1,000 for first sample,$400 for each additional up to 7 samples done concurrently

• Single read sequencing ( 18 or 36bp ): $1,000 per lane, $7,000 per flowcell (7 lanes)

• Single read sequencing ( 76bp ): $1,300 per lane, $9,000 per flowcell (7 lanes)

• Paired-end sequencing ( 18 or 36bp ): $1,900 per lane, $13,000 per flowcell (7lanes)

* Paired-end sequencing ( 76bp ): $2,300 per lane, $16,000 per flowcell (7lanes)

High Throughput DNA Sequencing (HTS) : On-campus service fees based upon sequencing of 12 or more 96-well plates within a 3-month period. No more than 2 primers are allowed per project for HTS Full Service and HTS No Prep projects.  For projects that do not meet the minimum plate requirements, or those with more than 2 primers, please consult the DNA Core Sequencing pages for Core and Medium Throughput services and prices. For descriptions and guidelines for Sanger sample submission, please visit our Sanger Sequencing Services Page.

  HTS Full Service:  Culture, prep, reactions, clean-up, loading on 3730xl, data pickup site:

• Full service HTS is through one of two options for template preparation:

1. Heat lysis DNA template preparation

• $144.00/96-well plate for first primer.
• $124.80/96-well plate for second primer on same template.

2. Filter plate DNA template preparation

• $216.00/96-well plate for first primer.
• $124.80/96-well plate for second primer on same template.

Overall, the project sequencing success rate is nearly equivalent for both
procedures, but the sequence read lengths average approximately up to 100bp longer with the
filter prepared template versus heat lysis template preparation options.

HTS No Prep:  Reactions, clean-up, loading on 3730xl, data pickup site.

• $124.80/96-well plate for first primer, same price for second primer if desired.

HTS BAC-end Sequencing:

• Full service BAC-end Sequencing is $240.00/96-well plate
• Sequencing from a purified BAC(no prep) or second primer is $153.60/96-well plate

  HTS Ready-to-Load (RTL) plus Clean-up:  Clean up, loading on 3730xl, data pickup site.

• $80.00/96-well plate.

  HTS RTL: Loading on 3730xl, data pickup site.

• $75.00/96-well plate

  Colony Picking:  Uses Genetix Qpix II robotic colony picker.

• $150 set-up fee per library plus $0.10 per clone.
• $8.00 for duplicate plates (96-well or 384-well).

  Rearraying/Reracking:  Uses Genetix Qpix II robotic rearrayer.

Creates a new subset of plates from previously sequenced plates (useful for 3’ sequencing of plasmid clones or preparation for template amplification for microarray printing).  The investigator will be provided with a detailed printout of clone ID numbers for each well.  Rearray fidelity is confirmed through sequencing.

• $450.00 set-up fee plus $0.60 per clone. Duplicate plates included at no charge.

 This is an abridged list of our service prices and subject to change at any time.  For further information please contact Dr. Alvaro Hernandez, Director of DNA Services ( aghernan@illinois.edu ) at 217-244-3480 or Chris Wright, Assistant Director of DNA Services ( clwright@illinois.edu ) at 217-333-4372 to discuss ways the staff can be of assistance in achieving your project goals or to receive a quote for your project.  

The full fee applies to all wells left empty on a plate.  If the percentage of successful reactions on a plate is below 80% (defined as 200 high quality bases) due to a problem deemed to be a technical issue in the Keck Center, the price per plate will be adjusted proportionally downward.  Sequencing success rates will be calculated for each plate and those with success rates of 30% and above will be billed.  The investigator will be billed base on the combined success rates of the original and the repeated plates. 

Facility users other than State of Illinois institutions are subject to a 25.3% indirect cost rate for federally funded projects. For off-campus projects a contract must be negotiated through University of Illinois Grants and Contracts.  For further information contact Dr. Mark A. Mikel (217-244-0144; mmikel@illinois.edu ), Associate Director of the Roy J. Carver Biotechnology Center.