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Custom Oligonucleotide
Synthesis |
Four synthesis scales are available in Custom productions: the 40 nmol, .2
umol, 1 umol, and 10 umol. All synthesis scale samples are processed as Crude.
The 40 nmol and .2 umol scales can be purified using OPC or PAGE purification.
For description of Crude vs. Purified oligos, please check out our Crude
Oligo page. Oligos up to 150 bases in length may be synthesized. Standard degenerate bases
are accepted at no extra charge and must follow IUB nomenclature. Non standard
degenerate bases may be made at an extra charge of $10.00 per mix. A number of
special bases and oligo modifications available. Please check out our special
modifications page which lists many of our more common requests. Prices vary
for individual projects, so please call for more information.
Custom oligos cannot be ordered over the net at this time. A downloaded
form may be emailed to gen-engr@uiuc.edu or faxed to us at , 217-265-5066 respectively,
or you may fill out a form at our office in room 334/340 ERML.
 Download Custom Oligo Order Form (Word format
or
pdf format)
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40 nmol scale |
Avg.
Min. Yield
(ug) |
Avg. Turn
Around Time |
.2 umol scale |
Avg.
Min. Yield
(ug) |
Avg. Turn
Around Time |
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< 50 bases
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available |
132 |
24 hrs |
available |
660 |
24 hrs |
| 51-80 bases |
available |
132 |
24 hrs |
available |
660 |
24 hrs |
| 81+ bases |
N/A |
- |
- |
available |
660 |
24 hrs |
| OPC Purif. |
available |
66 |
24-48 hrs |
available |
330 |
48-72 hrs |
| PAGE Purif. |
available |
30 |
48-72 hrs |
available |
120 |
72-96 hrs |
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1 umol scale |
Avg.
Min. Yield
(mg) |
Avg. Turn
Around Time |
10 umol scale |
Avg.
Min. Yield
(mg) |
Avg. Turn
Around Time |
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< 20 bases
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available |
3.3 |
96 hrs |
available |
33 |
96 hrs |
| 21-50 bases |
available |
3.3 |
96 hrs |
N/A |
- |
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| IUB Nomenclature |
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Special Modifications
Following is a partial list of some of the more common modifications.
Please call for more information on your specific needs and a price
quotation.
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Methyl Phosphonamidites
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Methyl Phosphoramidites
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Phosphorothioate Linkages
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Chain Terminators
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Halogenated Nucleosides
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Fluorescently Labeled Oligos
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5' and 3' Amino modifiers
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Chemical Phosphorylation
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Biotin Labeling
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Cyanine Labeling
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Modified RNA Synthesis
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| Base |
Code |
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| A |
A |
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| G |
C |
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| C |
G |
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| T |
T |
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| (AC) |
M |
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| (AG) |
R |
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| (AT) |
W |
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| (CG) |
S |
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| (CT) |
Y |
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| (GT) |
K |
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| (AGC) |
V |
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| (ACT) |
H |
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| (AGT) |
D |
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| (CGT) |
B |
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| (AGCT) |
N |
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Please contact the facility for our very competitive
prices at (217) 244-1007. Oligos can be delivered via campus mail to the user.
High-Throughput Sequencing and Genotyping Unit
Virginia Lukas- Research Specialist
334 Edward R. Madigan Laboratory, 1201 W. Gregory
Drive, Urbana, IL 61801
Phone: (217) 244-1007 FAX:
(217) 265-5066 Email:vlukas@uiuc.edu
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Last edited:
29 Nov 2004
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