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Sample analysis using the new LIZ500 5-dye system.
Fragment Analysis
DNA fragment analysis is performed on the
ABI Prism 3730xl Analyzer, a capillary electrophoresis platform that
provides accurate size calling, and higher reproducibility.
Our facility can analyze fragments from 50-1000bp in size from many different genotyping applications.
Size Standards
Several size standards are available to analze fragments with 3-dye or 4-dye multiplexing. Size standards are provided by our facility and do not need to be purchased separately. Please call if you would like to use a custom size standard.
| Size Standard |
Size Range (bp)
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Blue Dye Label |
Green Dye Label |
Yellow Dye Label |
Red Dye Label |
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LIZ120
|
50-120
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dR110
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dR6G
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dTAMRA
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dROX
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ROX400
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50-400
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6-FAM
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HEX
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NED
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ROX500 |
50-500 |
6-FAM |
HEX |
NED |
PET |
LIZ500 |
50-500 |
6-FAM |
VIC |
NED |
|
LIZ600 |
50-600 |
6-FAM |
VIC |
NED |
PET |
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ROX1000
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50-1000
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6-FAM
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HEX
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NED
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- |
Pricing and Submission Information
Pricing is dependent on the size of your project. Fees include size standard,
electrophoresis on the ABI 3730xl and access to GeneMapper analysis software.
We provide one free 96-well test plate of serial dilutions to determine optimal DNA concentrations for each new customer.
Project Size |
Univeristy of Illinois
Urbana-Champaign |
Other Illnois Universities |
Out-of-state universities, government facilities, private companies or international laboratories |
Low-Throughput
1-95 samples |
$2.94 ea |
$3.23 ea |
$3.68 ea |
Medium-Throughput
1-11 96-well plates
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$100.00/plate |
$110.00/plate |
$125.30/plate |
High-Throughput
12+ plates in a 3 mo. period |
$100.00/plate |
$110.00/plate |
$125.00/plate |
For questions about choosing size standards and primer dye labels, for order submission and obtaining access to software
for analysis, please check out the Fragment Analysis FAQ
or contact Ms. Laura Guest at lguest@illinois.edu or 217-333-9520
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High-Throughput Sequencing and Genotyping
Unit
Laura Guest - Senior Research Specialist
334 Edward R. Madigan Laboratory, 1201 W. Gregory Drive, Urbana, IL 61801
Phone: (217) 333-9520 FAX: (217) 265-5066
Email: lguest@illinois.edu
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| Last edited:
19 November 2009 |
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