Cell Cycle Analysis
Cell cycle analysis is a very common application of Flow Cytometry. By using a DNA-specific stain one can determine the DNA profile e.g. find percentage of the population in G0/G1, S, and G2/M. This information can be used for example to monitor the effect of the anticancer treatment.
Below are some examples of cell cycle data acquired using a BD LSR II flow cytometer with FCS Express software analysis and this same data using mathematical modeling using ModFif software.
FCS Express Analysis
Diploid |
100% |
Diploid G1 |
82% |
Diploid G2 |
6.72% |
Diploid S |
11.28% |
G2/G1 |
1.90 |
Modfit Analysis
(with singlet discrimination)
Diploid |
100% |
Diploid G1 |
79.77% |
Diploid G2 |
4.57% |
Diploid S |
15.65% |
G2/G1 |
1.92 |
Common Cell Cycle Dyes Used at Facility
Probes |
Facility Instruments |
Excitation laser line |
Emission Filter [nm] |
Propidium Iodide
{Dead Cells} |
BD LSR II
BD FACSCanto
Coulter Epics XL
MoFlo |
488 nm
488 nm
488 nm
488 nm |
695/40
670 LP
630 BP
630/40 |
Hoechst
{Live Cells} |
BD LSR II
MoFlo |
407 nm
351 nm |
450/50
450/65 |
7 Aminoactinomycin D (7AAD)
{Dead Cells} |
BD LSR II
BD FACSCanto
Coulter Epics XL
MoFlo |
488 nm
488 nm
488 nm
530 nm |
695/40
670 LP
630 BP
630/40 |
DAPI
{Dead Cells} |
BD LSR II
MoFlo |
407 nm
351 nm |
450/50
450/65 |
DRAQ5
{Live Cells} |
MoFlo |
647 nm |
675 /30 |
| Flow Cytometry Facility
Barbara Pilas, Ph.D - Director
231 Edward R. Madigan Laboratory, 1201 W. Gregory Drive, Urbana, IL 61801
Phone: (217) 244-0559 FAX: (217) 244-0446 Email: http://uiuc.edu/
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| Last edited:05 June
2007
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