Flow Cytometry Facility - Immunophenotyping

Immunophenotyping

DIRECT STAINING
Fluorescent probe attached to primary antibody

Protocols for Direct Immunofluorescence Staining with Flow Cytometry

Abd Serotec
abcam

INDIRECT STAINING
Fluorescent probe attached to a secondary antibody

Protocols for Indirect Immunofluorescence Staining with Flow Cytometry

abcam
Abd Serotec

AVIDIN-BIOTIN METHOD
Biotynylated antibody can bind many avidins (streptavidins) - fluorochromes complex to improve sensitivity of assay

Protocols for Avidin-Biotin Staining with Flow Cytometry

Sigma Aldrich

Zenon® labeling technology

Zenon technology is based on the complexation of primary antibodies with dye– or enzyme–labeled Fab fragments of secondary antibodies directed against their Fc regions.
Molecular Probes

General Staining Techniques

Vendors

Flow Cytometry Facility
Barbara Pilas, Ph.D - Director
231 Edward R. Madigan Laboratory, 1201 W. Gregory Drive, Urbana, IL 61801
Phone: (217) 244-0559 FAX: (217) 244-0446 Email: http://uiuc.edu/

Last edited: 14 May 2007