The campus rate for peptide antigen determination is $40 per peptide. However, if a client uses his peptide to make an antibody at the IRC, we offer free peptide analysis. We don't provide peptide synthesis service but we can order peptides for clients from Protein Science Facility (PSF) at the UIUC. Most of our antibody production peptides are made by the PSF. For peptide synthesis information, please check PSF website at (http://www.biotech.uiuc.edu/centers/Proteomics/Proteinscience/index.html).

If a client prefers to use other sources to make his peptides, make sure that the following requirements are met:

1. Peptides have to be equal or longer than 8 amino acids. Twelve to fifteen amino acids peptides are preferred.
2. Peptides containing non-terminal cysteine should be avoided.
3. A cysteine residue must be present at either N-terminus or C-terminus for conjugation chemistry.
4. A peptide should contain amino N-terminus and amide C-terminus.
5. Five milligrams of peptides are required for a project.
6. Peptide purity has to be equal to or larger than 75%.
7. Peptides have to be provided with MS analysis and HPLC purification data.
8. Peptides should be dehydrated and stored at -20C after they are synthesized.

Immunological Resource Center Liping Wang, Laboratory Supervisor 264D Burrill Hall, 407 South Goodwin Avenue, Urbana, IL 61801 Phone: (217) 244-0558     FAX: (217) 244-0557    Email: IRC@life.uiuc.edu

Last edited: 10 Oct 2005