Immnological Resource Center
Service fees
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The fees presented here are for university of Illinois users only for academic year 2009-2010 starting on July 1 st, 2009. Non-UI clients will be charged additional overhead fee of 25.3 % for every service provided.
A. Polyclonal Antibody Generation
Animals (species)
|
Antigen quantity required |
*Antigen purity required
|
Testing sample
|
Final product
|
Time (weeks) |
Fees
|
Rabbit
(NZW) |
1.5mg for one animal |
90% |
2 x 10ml serum from each animal |
70ml serum from each animal |
2-5 months |
$598 (use 1 animal)
|
Chicken
(farm chicken) |
1.5mg for one animal |
90% |
500ul serum
|
10 eggs |
3-4 months |
$250 ( use 1 animal)
|
*Less than 90% purity is acceptable under certain conditions.
B. New Monoclonal Antibody Generation from Mouse
Animal |
Antigen quantity required |
Antigen purity required |
Time frame |
Products |
Fees
|
Balb/c mouse
(5 female) |
1.5mg at 1-
2mg/ml |
>80% |
4-6 months |
Hybridoma cell lines and medium |
$1,910 |
C. Monoclonal Antibody Production with Existing Hybridoma Cells
Production
Methods |
Products
|
Antibody concentration
|
Time frame |
Fees
|
Conventional flaks culture |
100ml
hybridoma medium |
0.02 -0.1mg/ml |
5-7 days |
$0.5/ml |
Integra CL350 bioreactor |
36ml
hybridoma medium |
~1mg/ml |
9 weeks |
$590 |
Integra CL100
Bioreactor |
90ml
hybridoma medium |
~1mg/ml |
9 weeks |
$899 |
Mouse ascites |
Crude ascites fluid |
20ml
1-6mg/ml
|
9 weeks |
$400 |
Notes:
Hybridoma medium products contain a small quantity of bovine antibody and other serum proteins introduced from FBS-containing culture medium. Serum-free medium production is available for conventional flask production at quotation. Mouse ascites also contains small quantity of mouse host antibody.
D. Antibody Purification
Service |
Starting material |
Fee |
Time
Frame
|
IgG purification with Protein A |
5ml Rabbit serum |
$450 for 20mg of purified antibody |
1-3 weeks |
IgG purification with Protein G |
20 ml Mouse Ascited
|
$450 for >20mg of purified antibody
|
100ml hybridoma medium |
$295 for 3-5mg purified antibody |
Antigen affinity purification |
Serum/ascites |
Ask for quotation |
IgM purification |
Serum/ascites |
Ask for quotation |
E. Miscellaneous Antibody Services
Service |
Quantity |
Fee |
ELISA |
One 96-well plate |
$25 |
Antibody isotyping |
One cell line |
$241 |
Peptide antigen design |
One Protein Analysis |
$402 |
Peptide conjugation to KLH |
One peptide |
$70 |
Antibody biotinylation |
One protein (1-10mg) |
$50 |
1discount given to multiple cell line isotyping, ask for quote.
2 The fee is weaved for antibody production with the peptide picked.
F. Biacore 3000 Services
Service |
Fee
University of Illinois |
Instrument individual training |
$100 |
Instrument usage hourly rate
|
$35
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For Biacore training and more information on Biacore service, please contact lab supervisor Liping Wang at
217-244-0558 or lpwang@life.uiuc.edu. For Biacore technical information and products, please visit http://www.biacore.com.
Facility users other than State of Illinois institutions are subject to a 25.3.% indirect cost rate for federally funded projects. For non-federally funded and commercial projects the indirect cost rate is 30%. For off-campus projects exceeding $5,000 a contract must be negotiated through University of Illinois Grants and Contracts. For further information contact Dr. Mark A. Mikel (217.244.0144, mmikel@uiuc.edu), Associate Director of the Biotechnology Center.
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