Protein Analysis by MALDI-TOF Mass Spectrometry

Guidelines for Sample Preparation

General sample handling  

To minimize protein losses from adsorption to walls of tubes, use polypropylene tubes or siliconized polypropylene tubes. Avoid polystyrene (clear plastic) and glass.
Use freshly prepared, high purity reagents and water. Contaminants from buffers, detergents, urea, guanidine can prevent a good mass spectrometry result.

Sample submission

• In solution, as concentrated as possible, volume 10-20 ul
• Minimum Concentration 10 pmol/microliter
• Use 200ul PCR-style eppendorf tubes

Peptide Synthesis and Purification | Protein Sequence Analysis 
Mass Spectrometry | 2D Gel Electrophoresis

Protein Sciences Facility
Peter Yau, Ph.D - Director
315 Noyes Laboratory, 505 South Mathews Avenue, Urbana, IL 61801
Phone: (217) 333-4695     FAX: (217) 244-1142      
Email: proteinsciences@uiuc.edu