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 Carver Biotechnology Center
Protein Sciences Facility
Pricing
Effective July 2008

State of Illinois institutions are subject to a 10% indirect cost surcharge. All other users are subject to a 25.3% indirect cost surcharge.  For off-campus projects exceeding $2,000 a contract must be negotiated through University of Illinois Grants and Contracts.  For further information contact Dr. Mark A. Mikel (217.244.0144, mmikel@illinois.edu), Associate Director of the Roy J. Carver Biotechnology Center.

Peptide Synthesis On-campus Ill inst. & State Gov. Off-campus
0.1mmol scale/aa $ 27.50 $ 30.25 $ 34.46
0.25mmol scale/aa $ 45.00 $ 49.50 $ 56.39
Special Modifications $ 110.00 $ 121.00 $ 137.83 Minimum*
Cyclizations $ 275.00 $ 302.50 $ 344.58 Minimum*
Consultation 50/hr. minimum $ 50.00 $ 55.00 $ 62.65
HA Peptide/mg (In 10mg quantities) $ 30.00 $ 33.00 $ 37.59
FLAG Peptide/mg (In 10mg quantities) $ 15.00 $ 16.50 $ 18.80
cMYC Peptide/mg (In 10mg quantities) $ 15.00 $ 16.50 $ 18.80
Minimum charge $ 220.00 $ 242.00 $ 275.66
*Additional cost may apply
Chromatography
Analytical HPLC/inj (4.6mm X 100mm) $ 55.00 $ 60.50 $ 68.92
Fraction Collection $ 30.00 $ 33.00 $ 37.59
Purification/inj (RP, Maximum of 100mg crude) $ 225.00 $ 247.50 $ 281.93
FPLC (MonoQ 5/5 and Mono S 5/5 with fraction collection) $ 140.00 $ 154.00 $ 175.42
MDLC (off-line Ion-exchange) $ 150.00 $ 165.00 $ 187.95

Proteomic Services
Isoelectric focusing
11 cm strip $ 55.00 $ 60.50 $ 68.92
24 cm strip $ 65.00 $ 71.50 $ 81.45

SDS Gel electrophoresis
Criterion® (13 cm X 9cm X 1.0mm) $ 55.00 $ 60.50 $ 68.92
Dalt® (24cm X 20cm X 1.0mm) $ 230.00 $ 253.00 $ 288.19
Custom gel Inquire

Gel Staining
Coomassie R-250      
Criterion® $ 22.50 $ 24.75 $ 28.19
Dalt® $ 55.00 $ 60.50 $ 68.92
Sypro Ruby®      
Criterion® $ 35.00 $ 38.50 $ 43.86
Dalt® $ 275.00 $ 302.50 $ 344.58

Imaging (Typhoon® 9400)
Self-service 1st half hour $ 25.00 $ 27.50 $ 31.33
Each add'l half hour $ 20.00 $ 22.00 $ 25.06

Electroblotting on PVDF
Mini (8cm X 7cm X 1.0mm) $ 55.00 $ 60.50 $ 68.92
Criterion® $ 125.00 $ 137.50 $ 156.63

Trypsin Digestion
Solution $ 80.00 $ 88.00 $ 100.24
add'l solution samples $ 65.00 $ 71.50 $ 81.45
In-gel $ 150.00 $ 165.00 $ 187.95
add'l gel samples $ 115.00 $ 126.50 $ 144.10

N-terminal Sequencing
Set-up fee with five residues $ 250.00 $ 275.00 $ 313.25
Per residue $ 27.50 $ 30.25 $ 34.46
Amino Acid Analysis in Duplicate $ 150.00 $ 165.00 $ 187.95
Amino Acid Analysis in Triplicate $ 200.00 $ 220.00 $ 250.60

Mass Spectrometry
Maldi $ 30.00 $ 33.00 $ 37.59
Direct infusion $ 30.00 $ 33.00 $ 37.59
LC-MS with Absorbance $ 135.00 $ 148.50 $ 169.16
LC-MS $ 110.00 $ 121.00 $ 137.83
MS data analysis by PEAKS® $ 75.00 $ 82.50 $ 93.98
Custom Database setup $ 100.00 $ 110.00 $ 125.30
Detailed Data Analysis (or data archival retrieval)/hr $ 100.00 $ 110.00 $ 125.30

Sample preparation/sample
Lyophilization (<5ml) $ 20.00 $ 22.00 $ 25.06
Lyophilization (5-200ml) $ 30.00 $ 33.00 $ 37.59
2D clean-up/zip-tip® $ 12.50 $ 13.75 $ 15.66

Electrophoresis (DIGE®)
Labeling (Cy-Dye®) $ 600.00 $ 660.00 $ 751.80
Imaging $ 75.00 $ 82.50 $ 93.98
Spot Analysis/hr-2hr minimum (Decyder®/Samespots®) $ 50.00 $ 55.00 $ 62.65
Spot Handling (Digestion/extraction) $ 250.00 $ 275.00 $ 313.25
Maldi Analysis (robot spotted, PMF)/sample $ 50.00 $ 55.00 $ 62.65
LC-MS/sample (includes data analysis) $ 110.00 $ 121.00 $ 137.83

Protein Sciences Facility
Peter Yau, Ph.D - Director
315 Noyes Laboratory, 505 South Mathews Avenue, Urbana, IL 61801
Phone: (217) 333-4695     FAX: (217) 244-1142      
Email: proteinsciences@illinois.edu