The Transgenic Mouse Facility will perform experiments to clean a mouse line from pathogen infections. The procedure will include superovulating, mating and collecting fertilized 1-cell embryos. The investigator is responsible for all the mouse purchase. Females are super-ovulated and bred to males of the line to be rederived. Embryos are collected and pooled, washed to further reduce the risk of pathogen transfer, and then surgically transferred, using aseptic technique, into the reproductive tract of pseudopregnant SPF females. Upon weaning of the pups, DAR will submit surrogate dams for diagnostic testing, including screens for bacterial and viral pathogens.
To request service from Transgenic Mouse Facility:
- Schedule an appointment with facility Director, Fuming Pan (244-0649, fpan@uiuc.edu) to discuss the rederivation project.
- Complete and submit the Service Request Form for mouse line rederivation.
- For each line to be rederived, the investigator should provide 5-10 proven breeder males, 8-9 weeks of age and 10 females, 3-4 weeks of age.
- The investigator will perform the hormone injections, breeding setup. The TMF staff will perform embryo harvest, washes and embryo transfers.
- All the animal care costs and per diem are the responsibility of the investigator.
About TMF Services & Fees
Request Form Transgenic Gene Targeting Knockout Cryopreservation Rederivation
Mouse Embryo Recovery
Transgenic Mouse Facility
Fuming Pan, Director
505 S. Goodwin Ave., 15 Morrill Hall, Urbana, IL 61801
Phone: (217) 244-0649 FAX: (217) 244-0466
Email: fpan@uiuc.edu
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