Transgenic Mouse Production

Transgenic mice are produced by injecting the DNA of interest into one-cell mouse embryos followed by implantation into foster mice. The facility will purify the transgene insert from a plasmid vector and microinject the purified DNA into mouse embryos. The gestation period of a mouse is approximately 20 days. Pups will be transferred to the investigator for genotyping at 3 weeks of age.

Time line for transgenic mouse production:

Day 1	Day 21	Day 42 →
DNA injection	Pups Born	Wean & transfer pups	Breeding

To request service from Transgenic Mouse Facility:

• Provide the facility with DNA construct approval date from the Institutional Biosafety Committee (IBC). The facility will not initiate any experiment without the relevant approval date.

• Obtain approved protocol for animal research from IACUC. The facility will not initiate any experiment without an authorized animal research protocol number.

• Arrange an appointment with the facility Director, Fuming Pan (244-0649, fpan@uiuc.edu) to discuss and initiate the project.

• Complete and submit the Service Request Form for pronuclear microinjection, provide the facility with 20ug of digested plasmid DNA at 0.5ug/ul or higher with a picture of the DNA prepared for fragment isolation.

• The TMF will use C57BL/6 or B6C3F1 mice as egg donors, unless specially requested by investigator

• The TMF will provide at least 2 transgenic founder mice per construct.

• Investigators can purify their DNA inserts. We do not guarantee any transgenic mouse production using the purified DNA directly from investigators.

• All the offspring in the Transgenic Mouse Facility and all the cage cost must be transferred to the investigator’s account at the weaning day

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