The Transgenic Mouse Facility (TMF) at University of Illinois at Urbana-Champaign is one of the service cores in the Roy J. Carver Biotechnology Center. The mission of this state-of-the-art facility is to support research projects by providing genetically engineered mouse models to all investigators on campus. Services in the facility include transgenic mouse production by pronuclear microinjection of DNA constructs, mouse embryonic stem cell gene targeting, knockout mouse production by blastocyst microinjection, mouse embryo cryopreservation, and mouse line rederivation.